MOLECULAR CHARACTERIZATIONS OF CUCUMBER MOSAIC VIRUS AND TOMATO MOSAIC VIRUS ISOLATES IN HAKKARI TURKEY

Akdura N.

FRESENIUS ENVIRONMENTAL BULLETIN, vol.31, no.08B, pp.8987-8998, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 31 Issue: 08B
  • Publication Date: 2022
  • Journal Name: FRESENIUS ENVIRONMENTAL BULLETIN
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.8987-8998
  • Hakkari University Affiliated: Yes

Abstract

ABSTRACT

In this study, a total of 184 leaf samples were collected from tomato (Solanum lycopersicum L.) and pepper (Capsicum annuum L.) plants that showed virus-like symptoms. Sample collection was carried out in Hakkari province in October, 2020. Cucumber mosaic virus (CMV) and Tomato mosaic virus (ToMV) factors were investigated by Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). DAS-ELISA test showed that 106 (57.60%) of the samples were infected with one or more viruses. For phylogenetic analyses, 10 samples (7 tomato and 3 pepper) which were positive for CMV and ToMV were tested using Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. Coat protein (CP) and replicate genes of Hakkari CMV and ToMV isolates were amplified by RT-PCR using specific primers. The resulting DNA sequences were deposited in NCBI GenBank (ToMV-Accession Nos: OM286731, OM286732, OM286733, OM286734, CMV-Accession No: OM418629). When the replicase and coat protein gene sequences of four ToMV isolates and one CMV isolate were compared with the isolates in GenBank, a nucleotide homology of 98.1-99.6% and 91.7-99.4% were found, respectively. Phylogenetic analysis revealed a strong similarity between Hakkari ToMV and CMV isolates and other Turkish isolates. Consistent with the molecular studies conducted in Turkey on CMV and ToMV, the isolates detected in this study also clustered in subgroup Ia. With this analysis, CMV and ToMV were serologically and molecularly detected for the first time in Hakkari and the first report for ToMV replicase sequences in infected tomato and peppers were submitted.