International Journal of Biotechnology and Biomaterials Engineering, cilt.3, sa.1, 2021 (Scopus)
Kefiran is a hydro-soluble polysaccharide, which can be isolated from kefir grains biomass. In the present study was characterised physicochemical and morphological properties of kefiran isolated from kefir grains biomass, obtaining from a local dairy company. High performance liquid chromatography analysis of monosaccharides revealed that kefiran was composed of glucose and galactose in an approximate ratio of 1.0:0.4 in nature. Infrared spectra of kefiran revealed the presence of carboxyl, hydroxyl, and amide groups, which corresponded to a typical heteropolymeric polysaccharide, which indicated a purified structure of isolated kefiran from kefir grains biomass. The scanning electron microscopy images of polysaccharide kefiran showed homogeneous morphology with porous and a sponge-like structure. The functional groups of kefiran can make it more attractive for many modifications.