Akademik Veri Yönetim Sistemi
ID HEALTH SCIENCE, cilt.4, sa.1, ss.83-89, 2026 (Hakemli Dergi)
Staphylococcus aureus, particularly methicillin-resistant strains (MRSA), remains a major cause of hospital-acquired infections worldwide. This study aimed to determine the prevalence of MRSA and methicillin-susceptible S. aureus (MSSA) among clinical isolates, evaluate their antimicrobial resistance profiles, and identify mecA positivity, inducible MLS<sub>B</sub> (D-test), and hVISA phenotypes.A total of 161 S. aureus isolates obtained from various clinical specimens at Hatay Mustafa Kemal University Hospital between August 2023 and July 2024 were retrospectively analyzed. Identification was performed using MALDI-TOF and VITEK-2 systems. Antimicrobial susceptibility testing, inducible MLS<sub>B</sub> detection (D-test), and hVISA screening were interpreted according to EUCAST guidelines. Methicillin resistance was confirmed by mecA gene detection via PCR.MRSA prevalence was 40%, beta-lactam resistance 63%, TMPSMX and macrolide resistance ranged from 25% to 30%, mecA positivity was 40%, inducible MLS<sub>B</sub> (D-test) was detected in 32%, and hVISA in 13% of isolates. Antibiotic resistance was higher among elderly patients and ICU admissions, whereas MSSA predominated among outpatients.MRSA isolates demonstrated multidrug resistance, representing a significant challenge in hospital infection control and empirical therapy. This study provides updated local data on MRSA prevalence, mecA positivity, D-test, and hVISA phenotypes, contributing to evidence-based antibiotic stewardship and infection control strategies. Continuous local surveillance, rational antibiotic use, and effective infection control strategies are crucial to prevent the spread of resistant S. aureus strains.