Biofilm formation in clinical staphylococcus aureus isolates

Ahmed Z., Özdemir K., Mohammed B. H., ERTAŞ M., Turnen I.

Fresenius Environmental Bulletin, cilt.29, sa.7, ss.6303-6314, 2020 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 7
  • Basım Tarihi: 2020
  • Dergi Adı: Fresenius Environmental Bulletin
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.6303-6314
  • Anahtar Kelimeler: Antibiotics resistance, Biofilm formation, Staphylococcus aureus
  • Hakkari Üniversitesi Adresli: Evet

Özet

In this study a total of one hundred and twenty samples were collected from patients who were admitted to West Erbil Emergency, Emergency, and Rizgary teaching Hospitals during the period from ' March 2017 to 20 May 2017. Samples collected from different clinical sources: 50% from burns, 32% from surgical wounds, 10% from dental carries, and 8% from urine samples. Isolates were identified using cultural, morphological, biochemical tests, and confirmed by VITEK2 compact system. Fifty isolates were identified as Staphylococcus aureus. Antibiotic Sensitivity test by disk diffusion method was done for all S. aureus isolates against 20 commonly used antibiotics and the resistance percentage. All isolates were tested for ß-lactamase production, and all of them showed a positive result. PCR technique was used for the detection ofmecA gene in S. aureus isolates and the results showed that 62% of isolates were mecA positive, while 38% of the isolates were mecA negative. All S. aureus isolates were tested for their in vitro ability for biofilm formation using two methods: Congo red agar method, and microtiter plate assay. PCR technique was also used to detect the presence of icaAD gene in S. aureus isolates and the results showed that 94% of the isolates harbor this gene, while 6% were icaAD negative.