DETERMINATION OF THE GENETIC VARIATION AMONG SOME TURKISH RICE (ORYZA SATIVA L.) CULTIVARS USING ISSR TECHNIQUE

Törün B., Sözen E.

JOURNAL OF ANIMAL AND PLANT SCIENCES, cilt.28, sa.6, ss.1766-1773, 2018 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 6
  • Basım Tarihi: 2018
  • Dergi Adı: JOURNAL OF ANIMAL AND PLANT SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1766-1773
  • Hakkari Üniversitesi Adresli: Evet

Özet

In this study, the genetic diversity levels of 28 different rice varieties developed in Turkey were determined by using

ISSR markers. Twenty different ISSR primers amplified 268 bands of which 217 were polymorphic. The band number for

each primer ranged from 5-22 with an average number of 13.4. Polymorphism percentages varied between 40.00% and

94.12% with an average of 80.97%. Polymorphism Information Content (PIC) values were between 0.274 and 0.374

with an average value of 0.347. Similarity values indicated that the most similar varieties were Efe and Hamzadere

(0.856) and the least similar varieties were Demir and Bafra Yıldızı (0.395). UPGMA analysis grouped 28 rice varieties

into two main clusters and showed that genotypes obtained from the same institute tend to cluster together. This implies the

possibility of natural cross-pollination between rice varieties grown in close fields. Our results indicated that the ISSR

technique is effective in studying genetic variation in Turkish rice cultivars. Data obtained from this study could be used in

the rice improvement programs.